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KMID : 0869020060090010076
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Journal of Korean Orthopaedic Research Society
2006 Volume.9 No. 1 p.76 ~ p.88
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Effects of 3-aminobenzamide (3-AB) on the alterations in primary afferent and spinal neurons induced by spinal nerve injury in rats
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Cho Woo-Jin
Cho Hyung-Sook
Hwang Se-Jin
Paik Doo-Jin
Lee Seo-Eun
Kim Tai-Seung
Cho Jae-Lim
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Abstract
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Purpose: The authors studied the effect of the 3-AB, an inhibitor of poly(ADP-ribose)polymerase (PARP), on the changes of primary afferents and spinal cord after spinal nerve injury.
Methods:The L5 and L6 spinal nerve of the rats were cut, and 3-AB (10 mg/Kg) was injected intraperitoneally once per day. The animals were sacrificed 3 days, 7 days, 14 days and 28 days after nerve injury. Binding of isolectin B4 (IB4) and immunohistochemistry (IHC) of CGRP for the changes in primary afferents, IHC of NK1 for sensory neurons, and of cleaved caspase 3 and NeuN for the apoptotic changes in spinal neurons were performed.
Results:Decreased binding of IB4 and immunoreactivity (IR) for CGRP, increase of IR for NK1, and cleaved caspase 3 in both neurons and glia in ipsilateral dorsal horn were observed after spinal nerve injury. These changes were attenuated, especially at between 3 days and 14 days, by administration of 3-AB.
Conclusion:It is suggested that inhibition of PARP by 3-AB may attenuate alterations of primary afferents and spinal neurons, at least in early stage, after spinal nerve injury.
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KEYWORD
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Spinal nerve injury, Primary afferent, Apoptosis, PARP, Spinal cord
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